Bacterial 16S amplicon 454 pyrosequencing of environmental samples (vertebrate faeces, sediment, soil, surface water) to assess the potential of next generation sequencing methods for health related microbial water quality monitoring

This study set out to evaluate the used next generation sequencing (NGS) approach in a representative and complex backwater catchment with importance as a drinking water resource. In a multi-compartment approach, water, faeces, soil, and sediment samples were investigated by 454 pyrosequencing of bacterial 16S rRNA gene community (V1-V2 region) to assess the capabilities of NGS methods (i) for the development and evaluation of environmental molecular diagnostics, (ii) for direct screening of the bulk bacterial communities, and (iii) for faecal pollution analysis in water.

Identifier
Source https://data.blue-cloud.org/search-details?step=~012FAF39E75BE99C396A829C86F173F6F8E438ADE4A
Metadata Access https://data.blue-cloud.org/api/collections/FAF39E75BE99C396A829C86F173F6F8E438ADE4A
Provenance
Instrument 454 GS FLX Titanium; LS454
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Contributor Vienna University of Technology
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Temporal Coverage Begin 2010-06-15T00:00:00Z
Temporal Coverage End 2011-05-10T00:00:00Z