Influence of temperature, pCO2, and dissolved nutrients on the oxidative stress response and the carbon metabolism of phytoplankton


We conducted a full-factorial lab experiment to study the individual and combined effects of temperature (18°C and 21°C), pCO2 (400 and 1000 ppm), and dissolved N:P ratio (16 and 25 molar) on the antioxidant capacity and carbon metabolism of the phytoplankton Phaeodactylum tricornutum (strain CCAP 1052/1A). The antioxidant response was assessed based on different biomarkers, including the contents of protective carotenoids (ß-Carotene, diadinoxanthin and violaxanthin), and by determination of antioxidant enzyme activities, and Malondialdehyde (MDA) as a biomarker for oxidative stress. We quantified the activity of Managanese Superoxide Dismutase (SOD-Mn), Glutathione S-transferase (GST), Catalase (CAT), Glutathione Peroxidase (GPx). As possible consequence of oxidative stress on metabolic pathways of carbon, we also quantified carbon fluxes by measuring rates of growth, respiration, dissolved organic carbon (DOC) exudation, and cellular organic carbon content and particulate phosphorus (PP). We also quantified the concentration of photosynthetic pigments chlorophyll a (Chl-a) and fucoxanthin.

Metadata Access
Creator Meunier, Cédric Léo ORCID logo; Moreno, Hugo ORCID logo; Rokitta, Sebastian D ORCID logo; Tremblay, Nelly ORCID logo; Boersma, Maarten (ORCID: 0000-0003-1010-026X); Groß, Elisabeth ORCID logo; Klip, Helena; Wiltshire, Karen Helen ORCID logo
Publisher PANGAEA
Publication Year 2022
Funding Reference Federal Ministry of Education and Research Crossref Funder ID 01LN1702A
Rights Creative Commons Attribution 4.0 International;
OpenAccess true
Resource Type Dataset
Format text/tab-separated-values
Size 630 data points
Discipline Earth System Research