Absolute abundances of dinoflagellate cysts in surface sediment samples from four sites: Lycopodium marker-grain method

DOI

Absolute abundances (concentrations) of dinoflagellate cysts are often determined through the addition of Lycopodium clavatum marker-grains as a spike to a sample before palynological processing. An inter-laboratory calibration exercise was set up in order to test the comparability of results obtained in different laboratories, each using its own preparation method. Each of the 23 laboratories received the same amount of homogenized splits of four Quaternary sediment samples. The samples originate from different localities and consisted of a variety of lithologies. Dinoflagellate cysts were extracted and counted, and relative and absolute abundances were calculated. The relative abundances proved to be fairly reproducible, notwithstanding a need for taxonomic calibration. By contrast, excessive loss of Lycopodium spores during sample preparation resulted in non-reproducibility of absolute abundances. Use of oxidation, KOH, warm acids, acetolysis, mesh sizes larger than 15 µm and long ultrasonication (> 1 min) must be avoided to determine reproducible absolute abundances. The results of this work therefore indicate that the dinoflagellate cyst worker should make a choice between using the proposed standard method which circumvents critical steps, adding Lycopodium tablets at the end of the preparation and using an alternative method.

Supplement to: Mertens, Kenneth Neil; Verhoeven, Koen; Verleye, Thomas; Louwye, Stephen; Amorim, Ana; Ribeiro, Sofia; Deaf, Amr S; Harding, Ian C; De Schepper, Stijn; González, Catalina; Kodrans-Nsiah, Monika; de Vernal, Anne; Henry, Maryse; Radi, Taoufik; Dybkjaer, Karen; Poulsen, Niels E; Feist-Burkhardt, Susanne; Chitolie, Jonah; Heilmann-Clausen, Claus; Londeix, Laurent; Turon, Jean-Louis; Marret, Fabienne; Matthiessen, Jens; McCarthy, Francine M G; Prasad, Vandana; Pospelova, Vera; Hughes, Jane E Kyffin; Riding, James B; Rochon, André; Sangiorgi, Francesca; Welters, Natasja; Sinclair, Natalie; Thun, Christian; Soliman, Ali; Van Nieuwenhove, Nicolas; Vink, Annemiek; Young, Martin (2009): Determining the absolute abundance of dinoflagellate cysts in recent marine sediments: The Lycopodium marker-grain method put to the test. Review of Palaeobotany and Palynology, Review of Palaeobotany and Palynology, 157(3-4), 238-252

Identifier
DOI https://doi.org/10.1594/PANGAEA.754885
Related Identifier IsSupplementTo https://doi.org/10.1016/j.revpalbo.2009.05.004
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.754885
Provenance
Creator Mertens, Kenneth Neil ORCID logo; Verhoeven, Koen; Verleye, Thomas; Louwye, Stephen ORCID logo; Amorim, Ana ORCID logo; Ribeiro, Sofia ORCID logo; Deaf, Amr S ORCID logo; Harding, Ian C ORCID logo; De Schepper, Stijn ORCID logo; González, Catalina ORCID logo; Kodrans-Nsiah, Monika; de Vernal, Anne (ORCID: 0000-0001-5656-724X); Henry, Maryse; Radi, Taoufik; Dybkjaer, Karen; Poulsen, Niels E; Feist-Burkhardt, Susanne ORCID logo; Chitolie, Jonah; Heilmann-Clausen, Claus; Londeix, Laurent; Turon, Jean-Louis; Marret, Fabienne ORCID logo; Matthiessen, Jens ORCID logo; McCarthy, Francine M G; Prasad, Vandana; Pospelova, Vera; Hughes, Jane E Kyffin; Riding, James B; Rochon, André; Sangiorgi, Francesca ORCID logo; Welters, Natasja; Sinclair, Natalie; Thun, Christian; Soliman, Ali ORCID logo; Van Nieuwenhove, Nicolas ORCID logo; Vink, Annemiek ORCID logo; Young, Martin
Publisher PANGAEA
Publication Year 2009
Rights Creative Commons Attribution 3.0 Unported; https://creativecommons.org/licenses/by/3.0/
OpenAccess true
Representation
Resource Type Supplementary Publication Series of Datasets; Collection
Format application/zip
Size 4 datasets
Discipline Geosciences; Natural Sciences
Spatial Coverage (-16.670W, -9.934S, 2.930E, 54.690N); North Sea