Structure of Confined Albumin Layers

DOI

In the context of a project in collaboration with ISIS that focuses on structural studies under mechanical confinement, with a specific focus on biological lubricants, the applicants performed preliminary neutron reflectometry experiments at D17 (ILL) on the hydration of confined layers of mucins, the long glycoproteins that account for most of the material in biological fluids such as mucus and saliva. These experiments indicated that mucins can hold large amounts of water up to pressures as high as 5 bar. However, further controls are needed to support this interpretation. Here, we apply for investigating how the data obtained for mucins differ from that of a compact globular protein i.e., albumin. This will confirm that our previous results do indeed originate from the long unstructured nature of mucins and not by an instrumental artefact.

Identifier
DOI https://doi.org/10.5286/ISIS.E.92918657
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/92918657
Provenance
Creator Mr Ashton Stone; Miss Hannah Boyd; Professor Rob Richardson; Dr Rob Barker; Dr Javier Sotres; Dr Juan Francisco Gonzalez; Dr Stuart Prescott; Dr Rebecca Welbourn
Publisher ISIS Neutron and Muon Source
Publication Year 2021
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2018-05-14T08:00:00Z
Temporal Coverage End 2018-05-18T09:03:34Z