Enzymatic analysis of carbohydrate metabolism in Strongylocentrotus purpuratus larvae during laboratory experiments with different pH treatments


Strongylocentrotus purpuratus (Stimpson 1857) originally collected in November 2022 from La Jolla, USA (Lat: 32.842674; Long: -117.257767) were held in flow-through tanks which were filled with water from Kiel Fjord adjusted to 31.5 psu in the Christian-Albrechts-Universität zu Kiel at 10 °C. For experimental characterization of carbohydrate digestion between September and November 2023 S. purpuratus larvae were generated by gently shaking adult males and females and held in climate chambers at 15°C and 31.5 psu up to 10 days under two different food conditions. Using RT-qPCR analysis, expression patterns of five glycosidase genes including an amylase, laminarinase, cellulase, xylanase and trehalase were analyzed against the housekeeping gene EF1a. Digestion of starch, laminarin, carboxymethylcellulose, xylan and trehalose were analyzed under different pHs, at different sampling points and under different temperature conditions during and before the reaction using the DNS method.

The attached tables contain the data collected in laboratory experiments at the Christian Albrechts University, Kiel between September and November 2023. Larvae of cultured S. purpuratus were cultured for up to 10 days for the data collection. Several F1 generations were generated for the experiments, which were treated independently of each other. The larval test organisms were cultivated in climate chambers at principally 31.5 psu, 15.5 °C and pH 8.2.

DOI https://doi.pangaea.de/10.1594/PANGAEA.968989
Related Identifier IsPartOf https://doi.pangaea.de/10.1594/PANGAEA.968977
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.968989
Creator Hildebrand, Jasper ORCID logo; Stumpp, Meike ORCID logo
Publisher PANGAEA
Publication Year 2024
Funding Reference German Research Foundation https://doi.org/10.13039/501100001659 Crossref Funder ID 441084746 https://gepris.dfg.de/gepris/projekt/441084746 Black box larval physiology - mechanisms of nutrient acquisition and energetics of invertebrate larvae in a changing ocean
Rights Creative Commons Attribution 4.0 International; Data access is restricted (moratorium, sensitive data, license constraints); https://creativecommons.org/licenses/by/4.0/
OpenAccess false
Resource Type Dataset
Format text/tab-separated-values
Size 264 data points
Discipline Earth System Research
Spatial Coverage (-117.258 LON, 32.843 LAT); La Jolla, USA