Effects of pH and ionic strength on stability and structure of lipid-polymer nanodiscs


Nanostructured lipid aggregates offer enormous potential as tools for membrane structural biology and biophysics. Styrene-Maleic Acid Lipid Particles have a number of attractive characteristics including stability, ease of preparation and cost. In this proposal we wish to probe the role of charge on both the polymer and the lipid species in determining disc size, shape and stability. In our initial studies we used pH 8, 200mM salt since these are standard solution conditions when the discs are used in membrane protein purification. Here we will perturb these conditions, which will alter both the extent of charge screening and the hydrophobicity of the stabilizing polymer. We will study SMALP size and shape as a function of lipid composition at selected pH and salt concentrations to enable us to determine the fundamental biophysical parameters that underlie membrane behaviour in the discs

DOI https://doi.org/10.5286/ISIS.E.24088958
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24088958
Creator Professor Karen Edler; Dr Ilaria Idini; Dr Cameron Neylon; Ms Eleni Shiko
Publisher ISIS Neutron and Muon Source
Publication Year 2015
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2012-05-24T08:12:43Z
Temporal Coverage End 2012-05-30T07:27:18Z