Long-term experiment (182 days) with the phytoplankton species Chaetoceros affinis and Emiliania huxleyi at three nutrient regimes: measurements of cell size and density

DOI

Measurements of cell size, cell density, and nutrient concentration in a long-term experiment (182 days) with the marine phytoplankton species Chaetoceros affinis and Emiliania huxleyi, each consisting of nine genotypes (http://roscoff-culture-collection.org/; Emiliania huxleyi: GC22: RCC6383, C42: RCC6376, C41: RCC6375, C47: RCC4546, C96: RCC6381, C91: RCC6380, C35: RCC6374, C30: RCC6371, C48: RCC6377, Chaetoceros affinis: B63: RCC6347, B67: RCC6348, B68: RCC6349, B74: RCC6350). The species were cultivated together at three different nutrient regimes (10 N, 20 N, 30 N) with increasing nitrate supply. Nutrient concentrations were added as 9.0 ± 0.7 (10 N), 19.6 ± 0.8 (20 N), or 29.9 ± 0.9 μmoL-1 (30 N) nitrate and 0.9 ± 0.1 μmoL-1 phosphate. Silicate concentrations were aligned to reflect a 4:1 N:Si ratio. Cultivation was done in a semi-continuous batch cycle system with transfer of 2.88e7 ± 1.57e7 µm³ biovolume into a new batch every 7 days. Prior to the experimental start, genotypes were separately acclimated to experimental conditions with 20 μmol L−1 nitrate for 7 days. Cell density and cell volume were measured at the end of each batch cycle for both species. Phosphate, Nitrate and Nitrite were measured at the end of batch cycles 1, 7, 13, and 26.

Identifier
DOI https://doi.pangaea.de/10.1594/PANGAEA.957873
Related Identifier IsPartOf https://doi.org/10.1594/PANGAEA.957930
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.957873
Provenance
Creator Matthiessen, Birte ORCID logo; Hattich, Giannina S I ORCID logo; Pulina, Silvia ORCID logo; Hansen, Thomas; Reusch, Thorsten B H ORCID logo; Hamer, Jorin ORCID logo
Publisher PANGAEA
Publication Year 2023
Funding Reference German Research Foundation https://doi.org/10.13039/501100001659 Crossref Funder ID MA5058/2-2 ; German Research Foundation https://doi.org/10.13039/501100001659 Crossref Funder ID RE1708/17-2
Rights Creative Commons Attribution 4.0 International; Data access is restricted (moratorium, sensitive data, license constraints); https://creativecommons.org/licenses/by/4.0/
OpenAccess false
Representation
Resource Type Dataset
Format text/tab-separated-values
Size 11673 data points
Discipline Earth System Research