Chloroflexus spp. and Sulfurihydrogenibium spp. in microbial mats of the Nakabusa hot spring, Japan


Microbial mats develop in a wide range of aquatic habitats, such as geothermal hot springs, hypersaline ponds, marine cold seeps or hydrothermal vents. The Nakabusa hot spring is located in the Nagano Prefecture, Japan (36.3875N, 137.75E), dense olive-green microbial mats develop in regions where the slightly alkaline, sulfidic effluent has cooled to 65°C. The microbial community of such mats was analyzed by focusing on the diversity, as well as the in situ distribution and function of bacteria involved in sulfur cycling. Microbial mat samples were kept in sterile plastic tubes (for molecular analysis) or glass bottles completely filled with hot spring water to avoid oxidation. Samples were transferred to the laboratory on ice and used for physiological experiments within 8h. Quantification of cell biovolumes was carried out based on images of mat sections hybridized with Sulfurihydrogenibium- and Chloroflexi-specific probes, and stained with DAPI. In situ hybridizations (CARD-FISH) of thin matsections showed a heterogeneous vertical distribution of Sulfurihydrogenibium and Chloroflexus. Sulfurihydrogenibium dominated near the mat surface (50% of the total mat biovolume), while Chloroflexus dominated in deeper layers (up to 64% of the total mat biovolume).

Supplement to: Kubo, Kyoko; Knittel, Katrin; Amann, Rudolf; Fukui, F; Matsuura, Katsumi (2011): Sulfur-metabolizing bacterial populations in microbial mats of the Nakabusa hot spring, Japan. Systematic and Applied Microbiology, 34(4), 293-302

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Metadata Access
Creator Kubo, Kyoko ORCID logo; Knittel, Katrin ORCID logo; Amann, Rudolf ORCID logo; Fukui, F; Matsuura, Katsumi
Publisher PANGAEA
Publication Year 2024
Rights Data access is restricted (moratorium, sensitive data, license constraints)
OpenAccess false
Resource Type Supplementary Dataset; Dataset
Format text/tab-separated-values
Size 24 data points
Discipline Earth System Research
Spatial Coverage (137.750 LON, 36.388 LAT); Japan