NaCl modifies biochemical traits in bacterial endophytes isolated from halophytes: Towards salinity stress mitigation using consortia

This research isolated 120 bacterial endophytes from six halophytes (Distichlis spicata, Cynodon dactylon, Eragrostis obtusiflora, Suaeda torreyana, Kochia scoparia, and Baccharis salicifolia) from which 11 were tolerant to 2.5 M NaCl. These halophiles were molecularly identified and characterized with or without NaCl conditions. Characterization was based on tests such as indole acetic acid (IAA), exopolysaccharides (EPS), and siderophores (SID) production solubilization of phosphate (P), potassium (K), zinc (Zn), and manganese (Mn) mineralization of phytate enzymatic activity (acid and alkaline phosphatase, phytases, xylanases, and chitinases) and mineralization/solubilization mechanisms involved (organic acids and sugars). The 11 halophiles were identified as Oceanobacillus sp. (1), Oceanobacillus sp. (2), Nesterenkonia sp. (3), Bacillus velezensis (4), Halobacillus sp. (5), Oceanobacillus sp. (6), B. velezensis (7) Halomonas sp. (8), B. pumilus (9), Pseudomonas sp. (10) and Oceanobacillus sp. (11). Biochemical responses of nutrient solubilization and enzymatic activity varied between bacteria and the presence of NaCl. Organic acids were involved in P-mineralization and nutrient solubilization. Tartaric acid was common in the solubilization of P, Zn, and K. Maleic and vanillic acid were only detected in Zn and K solubilization, respectively. Moreover, sugars appeared to be involved in the solubilization of nutrients fructose was the general sugar detected in solubilization tests. Therefore, these bacteria should be tested as a consortium to mitigate saline stress in glycophytes under a global climate change scheme that threatens to exacerbate soil salinity

Identifier
Source https://data.blue-cloud.org/search-details?step=~0122F24D813EF9E0A14628695CAB9EF6904AC31B71E
Metadata Access https://data.blue-cloud.org/api/collections/2F24D813EF9E0A14628695CAB9EF6904AC31B71E
Provenance
Instrument Illumina Genome Analyzer II; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Temporal Point 2022-08-15T00:00:00Z