Abundance of N-cycling genes in an oxygen-deficient upwelling system during La Niña (2018) and El Niño (2019)

DOI

Seawater samples were collected with 10-L Niskin bottles in four stations along a cross-shore transect in front of the Port of Mazatlán (Mexico) during the oceanographic cruises Maz IV (April 2018) and Maz V (April 2019) on board the R/V "El Puma" (UNAM). Samples for molecular analyses (abundance of N-cycling genes) were collected at different depths based on the oxygen and chlorophyll-a profiles: surface (5 m depth); mixed layer, deep chlorophyll maximum (at 10–45 m depth); oxycline, base of the oxycline (at 60–125 m depth); upper OMZ (only for Maz V); OMZ core (at 250 m depth); and bottom (between 23 m depth in S1 and 670 m depth in S4). Water samples were filtered on board through polycarbonate membranes (0.22-μm pore, Merck Millipore). Genomic DNA was extracted with the DNeasy PowerWater Kit (Qiagen). The abundance of N-cycling genes was assessed using the gene copy number of bacterial and archaeal amoA, anammox bacterial 16S rRNA, nirK, nirS, and nrfA, using a StepOnePlus real-time PCR system (Applied Biosystems).

Identifier
DOI https://doi.org/10.1594/PANGAEA.954155
Related Identifier IsSupplementTo https://doi.org/10.1111/1462-2920.16362
Related Identifier References https://doi.org/10.1594/PANGAEA.954152
Related Identifier References https://doi.org/10.1594/PANGAEA.954151
Related Identifier References https://doi.org/10.1594/PANGAEA.954089
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.954155
Provenance
Creator Pajares, Silvia ORCID logo; Merino, Martin; Farias, Laura ORCID logo
Publisher PANGAEA
Publication Year 2023
Funding Reference Agencia Nacional de Investigación y Desarrollo https://doi.org/10.13039/501100002848 Crossref Funder ID ICM2019-015 https://www.iniciativamilenio.cl/en/home_en/ Millennium Science Initiative Program; Consejo Nacional de Ciencia y Tecnología https://doi.org/10.13039/501100003141 Crossref Funder ID CF-2019-2266 Ciencia de Frontera
Rights Creative Commons Attribution-ShareAlike 4.0 International; https://creativecommons.org/licenses/by-sa/4.0/
OpenAccess true
Representation
Resource Type Dataset
Format text/tab-separated-values
Size 1620 data points
Discipline Earth System Research
Spatial Coverage (-106.702W, 23.000S, -106.428E, 23.154N); Port of Mazatlán
Temporal Coverage Begin 2018-04-22T00:00:00Z
Temporal Coverage End 2019-04-02T15:00:00Z