149 datasets found

The acute-to-chronic stage transition in Toxoplasma gondii involes a global restructuring of the parasite transcriptome. Prior work identified a single transcription factor...

To assess how the N,N di-aryl urea compound U21 effects Toxoplasma gondii tachyzoties, differential expression analysis via RNA-seq was utilized. Significantly upregulated and...

Comparative transcriptomes were performed to characterize the gene expression patterns of T. gondii tachyzoites after dinitolmide treatment.

Here we show that AP2XII-1 and AP2XI-2, two transcription factors expressed in tachyzoites, a stage that causes acute toxoplasmosis, silence genes specific to merozoites, a...

Molecular barcoding techniques have emerged as powerful tools to understand microbial pathogenesis. However, barcoding strategies have not been extended to protozoan parasites,...

Toxoplasma RNA (both tachyzoites and bradyzoites) was metabolically labeled with 4TU for 90 minutes followed by a chase. RNA was recovered 0, 30, 60, 120, 240, and 480 minutes...

Using a differentiation reporter strain, unstressed parasites (tachyzoites) or stressed, reporter positive parasites (bradyzoites) were isolated by flow cytometry and analyzed...

Whole genome sequencing of populations of T. gondii that are resistant to a member of the bicyclic azetidine compound series, BRD7929. The bicyclic azetidine series are...

Toxoplasma gondii secretes various virulence effector molecules into host cells to disrupt host interferon-? (IFN-?)-dependent immunity. Among the effectors, ROP18 directly...

The bromdomain protein TgBDP3 is associated with the TFIID transcriptioanl initiation complex in Toxoplasma gondii. To determine the genes that are regulated by TgBDP3, the...

The protozoan pathogen Toxoplasma gondii relies on tight regulation of gene expression to invade and establish infection in its host. The divergent gene regulatory mechanisms of...

Purpose: To shed light on the parasiticidal mechanisms of AN13762, we have adapted an original workflow which combines a forward genetic approach based on transcriptome...

To understand the genomic composition of T. gondii in Japan, we are sequencing the genome of Japanese isolates in our genomic sequencing project of T. gondii. As part of the...

There are two experiments included in this particular GEO accession: For the first, freshly excysted sporozoites of T. gondii VEG or H. hammondi Amer1 were put into culture for...

RNA-Seq and cut-tag data for Toxoplasma gondii parasites that conditional knockdown of the transcription factors AP2XII-1, AP2XI-2, and AP2V-2.

Auranofin, a reprofiled FDA-approved drug originally designed to treat rheumatoid arthritis, has emerged as a promising anti-parasitic drug. It induces the accumulation of...

Using an epitope-tagged BFD1-complemented strain, the genomic binding of BFD1 was assessed in alkaline-stress induced bradyzoites. Overall design: One experimental (anti-Ty) and...

Purpose: T. gondii is an obligate intracellular parasite that remodels host transcriptome during infection. How T. gondii provokes these changes is not fully understood. In the...

The goal of this project is to investigate the worldwide population structure of Toxoplasma gondii and the genome characterization of mutant T. gondii strains and strains...

Toxoplasma gondii persists in humans by converting from actively replicating acute stage tachyzoites to slow-growing chronic stage bradyzoites. The molecular mechanisms that...