17 datasets found

The gut microbiome has been identified as a key to immune and metabolic health, especially in industrialized populations. Non-industrialized individuals harbor more diverse...

Part of the Western acculturation project - Plantanal and D1-30 longitudinal babies from PR

This study aims to characterize the human skin facial microbiome at high resolution, covering the entirety of one side of the human face. High resolution spatial profiling has...

Our DNA extraction protocol is years old, and we want to compare new extraction methods to address outstanding issues. For more details, check out the motivation and...

Surgery is the only intervention for severe hidradenitis suppurativa (HS) that is potentially curative. Therefore, optimizing post-surgical care is imperative to improve patient...

The ongoing revolution in high-throughput sequencing continues to democratize the ability of small groups of investigators to map the microbial component of the biosphere. In...

MG_baby timeseries

Conventional wisdom holds that PCR amplification for sequencing should employ pooled replicate reactions to reduce bias due to jackpot effects and chimera formation. However,...

Comparison of different sample types using different primers and platforms

In this study, we compared the axillary microbiota profiles of Parkinson's disease patients with different cognitive impairment stages with healthy controls to assess whether...

A comparison of non-targeted (shotgun metagenomics) sequencing methods and targeted amplicon methods for the characterization of ancient human oral microbiomes.

Comparison of different sample types using different primers and platforms

In industrialized countries, non-communicable diseases have been increasing in prevalence since the middle of the 20th century. While the causal mechanisms remain poorly...

The American Gut project is the largest crowdsourced citizen science project to date. Fecal, oral, skin, and other body site samples collected from thousands of participants...

High-quality (HQ) reference genomes are essential for understanding the phylogeny and function of uncultured microbes in complex microbial ecosystems. However, existing...

combined samples sequenced with primers modified for forward barcoding on the 515f site, extended to include V5 and improved ITS

Xenomicrobiota from a variety of different environments were transplanted into germfree mice. Assembly and composition of the gut-selected communities were analyzed by...