Indo--1 Fluorescence and intracellular Ca2+ concentration in solution of Stylophora pistillata laboratory experiment

DOI

The fluorescence of the calcium dye Indo-1 was measured in solution by confocal microscopy at different calcium concentrations. A calibration curve was produced from the ratio of Indo-1 fluoresence at different calcium concentrations. A dissociation constant for indo-1 and calcium was derived from the calibration curve.

Identifier
DOI https://doi.pangaea.de/10.1594/PANGAEA.968789
Related Identifier IsSupplementTo https://doi.org/10.1242/jeb.247638
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.968789
Provenance
Creator Venn, Alexander A ORCID logo; Techer, Nathalie; Segonds, Natacha; Tambutté, Eric ORCID logo; Tambutté, Sylvie ORCID logo
Publisher PANGAEA
Publication Year 2024
Rights Creative Commons Attribution 4.0 International; Data access is restricted (moratorium, sensitive data, license constraints); https://creativecommons.org/licenses/by/4.0/
OpenAccess false
Representation
Resource Type Dataset
Format text/tab-separated-values
Size 191 data points
Discipline Basic Biological and Medical Research; Biology; Cell Biology; Life Sciences
Spatial Coverage (7.425 LON, 43.738 LAT)