A biophysical approach of studying the molecular interactions of a novel antibiotic with the Gram negative bacterial cell envelope.

DOI

Gram negative (-ve) bacteria possess numerous defenses against antibiotics, not the least of them being the intrinsic permeability barrier afforded by their outer membrane (OM) and various efflux mechanisms which pump out drugs. A novel compound was synthesized at the KCL which shows promising activity against Gram (-ves). However, there was a noticeable effect of drug efflux resulting in reduced efficacy. This project investigates novel formulations to increase OM permeability to the antibiotic. Drug/enhancer complexes were made, which pass into the cell and dissociate thereby circumventing intrinsic resistance and membrane-associated efflux mechanisms. Neutron reflectivity will offer a greater insight into the intimate molecular drug (pure and formulated)-membrane interactions in order to understand how drug uptake may be improved for achieving optimal antimicrobial activity

Identifier
DOI https://doi.org/10.5286/ISIS.E.86389096
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/86389096
Provenance
Creator Dr Olivia Pabois; Dr Maxmilian Skoda; Dr Cecile Dreiss; Miss Kalliopi-Kelli Vandera; Dr Richard Harvey
Publisher ISIS Neutron and Muon Source
Publication Year 2020
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2017-05-12T08:00:00Z
Temporal Coverage End 2017-05-15T08:01:40Z