Holocene sea ice and palaeoenvironmental conditions were reconstructed using three sediment cores from northeastern Baffin Bay (GeoB19948-3 and GeoB19927-3) and the Labrador Sea (GeoB19905-1) along a north-south transect based on sea ice IP25 and open-water phytoplankton biomarkers (brassicasterol, dinosterol and HBI III). In Baffin Bay, sea-surface conditions in the Early Holocene were characterized by extended (early) spring sea ice cover (SIC) prior to 7.6 ka BP. The conditions in the NE Labrador Sea, however, remained predominantly ice-free in spring/autumn due to the enhanced influx of Atlantic Water (i.e., WGC; West Greenland Current) from 11.5 to ~9.1 ka BP, succeeded by a period of continued (spring-autumn) ice-free conditions from 9.1 to 7.6 ka BP corresponding to the onset of Holocene Thermal Maximum (HTM)-like conditions. A transition towards reoccurring ice-edge and significantly reduced SIC conditions in Baffin Bay is evident in the Mid Holocene (~7.6-3 ka BP) probably caused by the variations in the WGC influence associated with the ice melting and can be characterized as HTM-like conditions. These HTM-like conditions are predominantly recorded in the NE Labrador Sea area shown by (spring-autumn) ice-free conditions from 5.9-3 ka BP. In the Late Holocene (last ~3 ka), our combined proxy records from eastern Baffin Bay indicate low in-situ ice-algae production, however, enhanced multi-year (drifted) sea ice in this area was possibly attributed to the increased influx of Polar Water mass influx and may correlate with the Neoglacial cooling. The conditions in the NE Labrador Sea during the last 3 ka, however, continued to remain (spring-autumn) ice-free with decreased biological carbonate production probably linked to decreased WGC strength and/or increased Arctic Water influx, coinciding with the Neoglacial cooling.