The Kinetics of Self-Exchange of a Protein at Solid/Liquid interfaces

DOI

The formation of a protein corona occurs on all surfaces exposed to biological systems and understanding of how this corona forms is vital for many aspects of medicine and for assessing potential dangers from nanoparticle exposure. The most fundamental process underlying exchange of different proteins in a mixture with the surface is self-exchange of any given protein with the surface. This can only be measured by neutron reflectometry using exchange of protonated and deuterated proteins. We have recently been able to test out the ILL deuteration facilities standard "protein for physicists", MPB, and we have found that it has surface properties fairly similar to those of HSA, one of the key proteins in protein corona formation. We therefore propose to study the self-exchange kinetics of MPB with three different solid/liquid surfaces, hydrophilic, hydrophobic and their intermediate

Identifier
DOI https://doi.org/10.5286/ISIS.E.24079176
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24079176
Provenance
Creator Professor Jeff Penfold; Dr Bob Thomas
Publisher ISIS Neutron and Muon Source
Publication Year 2012
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2009-12-11T09:06:49Z
Temporal Coverage End 2009-12-15T08:41:03Z