Cryopreservation of liver cell spheroids used in artificial liver bio-transplant: QENS study.

DOI

The development of new cryopreservation strategies has major potential in medicine, in particular for storage of biological matter. The detailed characteristics of the cooling and thawing processes has proven crucial for the survival of liver bio-transplant cells in research being done at UCL's Royal Free Hospital Liver Group. A specially designed protocol ensures that more than 75% of cells survive cooling followed by thawing. Currently there is no clear explanation as to why this happens, the hypothesis being that it relates to the formation of glass pockets within an ice matrix. Previous measurement at ISIS have allowed us to prove the existence of a larger glass to ice ratio with the special protocol that when quench. We ask to continue the study by a detailed structural (diffraction) and dynamical (QENS) characterisations of the ice formation and the glassy pockets respectively.

Identifier
DOI https://doi.org/10.5286/ISIS.E.101137019
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/101137019
Provenance
Creator Professor Barry Fuller; Dr Victoria Garcia Sakai; Dr Oleg Kirichek; Professor clare selden; Mr Maooz Awan
Publisher ISIS Neutron and Muon Source
Publication Year 2022
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2019-02-08T09:30:00Z
Temporal Coverage End 2019-03-01T22:11:04Z