STRUCTURE OF THE COMPLEX BETWEEN CYTOCHROME P450 REDUCTASE AND CYTOCHROME C

DOI

Cytochromes P450 play a key role in drug metabolism in man. Electrons are supplied to the cytochromes by NADPH through the flavoprotein NADPH-cytochrome P450 reductase (CPR). Electron transfer from CPR to cytochrome P450 is only possible in a membrane environment, but electron transfer to cytochrome c occurs at a comparable rate in solution, facilitating structural studies. We have identified mutants of CPR which bind cytochrome c more tightly than the wild-type, and have shown that, for at least some of these mutants, the CPR-cytochrome complex is sufficiently long-lived to be studied by solution scattering. We therefore plan to use SANS, with 2H-CPR and contrast-matching experiments, to study the cytochrome c complexes of CPR and of four tightly-binding mutants. This will allow us to obtain, for the first time, a structural model of the CPR electron transfer complex.

Identifier
DOI https://doi.org/10.5286/ISIS.E.24088607
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24088607
Provenance
Creator Professor Gordon Roberts; Dr Vincent Huang; Dr Jacqueline Warwick Ellis
Publisher ISIS Neutron and Muon Source
Publication Year 2015
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2012-03-24T07:07:00Z
Temporal Coverage End 2012-03-25T17:09:00Z