This data set contains Planktic/Benthic + Planktic (P/B+P) ratio, Total alkenones concentrations, Alkenones accumulation rate , Benthic foraminiferal accumulation rate (BFAR) and benthic foraminiferal abundance in GeoB3327-5.The Humboldt Current System (HCS) is characterized by high marine primary productivity, the dynamics of which are closely linked to climate variability. However, its changes beyond the last glacial cycle are virtually unknow due to a dearth of long sediment records. Here we present a 500 ka multi-proxy marine productivity reconstruction from the southernmost part of the HCS (~43º S). At these latitudes, changes in marine productivity have been typically attributed to glacial-interglacial changes in the South Pacific Subtropical High (SPSH) and Southern Westerlies Winds (SWWs).