Metatranscriptomic, metagenomic, and ampilcon sequencing from hydrothermal plume waters collected above the Moytirra vent system (Lat 45.4833 N, Lon -27.8500 W) in July 2021, aboard the M/V OceanXplorer. Vent plume seawater was collected with Niskin bottles mounted to a CTD rosette. In this study, we combine prokaryote-specific 16S SSU rRNA gene sequencing, eukaryote-specific 18S SSU rRNA gene sequencing, metatranscriptomics, and metagenomics to examine how hydrothermal vent plume community biodiversity and metabolic activities change with distance from the Moytirra hydrothermal vent.