Microbial community composition and bacterioplankton at time series station Helgoland Roads, North Sea


A process of global importance in carbon cycling is the remineralization of algae biomass by heterotrophic bacteria, most notably during massive marine algae blooms. Such blooms can trigger secondary blooms of planktonic bacteria that consist of swift successions of distinct bacterial clades, most prominently members of the Flavobacteriia, Gammaproteobacteria and the alphaproteobacterial Roseobacter clade. This study explores such successions during spring phytoplankton blooms in the southern North Sea (German Bight) for four consecutive years. The surface water samples were taken at Helgoland Island about 40 km offshore in the southeastern North Sea in the German Bight at the station 'Kabeltonne' (54° 11.3' N, 7° 54.0' E) between the main island and the minor island, Düne (German for 'dune') using small research vessels (http://www.awi.de/en/expedition/ships/more-ships.html). Water depths at this site fluctuate from 6 to 10 m over the tidal cycle. Samples were processed as described previously (Teeling et al., 2012; doi:10.7554/eLife.11888.001) in the laboratory of the Biological Station Helgoland within less than two hours after sampling. Assessment of absolute cell numbers and bacterioplankton community composition was carried out as described previously (Thiele et al., 2011; doi:10.1016/B978-0-444-53199-5.00056-7). To obtain total cell numbers, DNA of formaldehyde fixed cells filtered on 0.2 mm pore sized filters was stained with 4',6-diamidino-2-phenylindole (DAPI). Fluorescently labeled cells were subsequently counted on filter sections using an epifluores-cence microscope. Likewise, bacterioplankton community composition was assessed by catalyzedreporter deposition fluorescence in situ hybridization (CARD-FISH) of formaldehyde fixed cells on 0.2 mm pore sized filters.

DOI https://doi.org/10.1594/PANGAEA.860256
Related Identifier https://doi.org/10.1126/science.1218344
Related Identifier https://doi.org/10.7554/eLife.11888
Related Identifier https://doi.org/10.1594/PANGAEA.737388
Related Identifier https://doi.org/10.1594/PANGAEA.736644
Related Identifier https://doi.org/10.1594/PANGAEA.756613
Related Identifier https://doi.org/10.1594/PANGAEA.783570
Related Identifier https://doi.org/10.1594/PANGAEA.783571
Related Identifier https://doi.org/10.1594/PANGAEA.820157
Related Identifier https://doi.org/10.1594/PANGAEA.810745
Related Identifier https://doi.org/10.1594/PANGAEA.820206
Related Identifier https://store.pangaea.de/Publications/Teeling-etal_2012/Oligonucleotide-probes_Teeling-etal_2012.pdf
Related Identifier https://store.pangaea.de/Publications/Teeling-etal_2016/Oligonucleotide-probes_Teeling-etal_2016.pdf
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.860256
Creator Fuchs, Bernhard M ORCID logo; Bennke, Christin M; Reintjes, Greta ORCID logo; Kassabgy, Mariette; Amann, Rudolf I ORCID logo
Publisher PANGAEA
Publication Year 2016
Rights Creative Commons Attribution 3.0 Unported; https://creativecommons.org/licenses/by/3.0/
OpenAccess true
Resource Type Dataset
Format text/tab-separated-values
Size 5239 data points
Discipline Earth System Research
Spatial Coverage (7.900 LON, 54.188 LAT); German Bight, North Sea
Temporal Coverage Begin 2009-01-05T00:00:00Z
Temporal Coverage End 2012-06-20T00:00:00Z