Quantification of microbial cells and virus-like particles in the water column above Kermadec Trench


Water samples were collected using 7.5-Liter Niskin bottles and fixed with 25% electron microscopy graded glutaraldehyde (1% final concentration) and stored at -80°C until quantification by flow cytometry. Samples were measured in triplicates using a BD FACSCanto™ II flow cytometer, after staining with SYBR Green I (Brussaard 2004). The flow rate was 5–7 μl/min, as determined by BD Trucount™ Beads. The laser settings and gating examples can be found in the Supporting Information of Schauberger et al. (2021).

DOI https://doi.org/10.1594/PANGAEA.965925
Related Identifier https://doi.org/10.1002/lno.11711
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.965925
Creator Schauberger, Clemens ORCID logo; Middelboe, Mathias ORCID logo; Glud, Ronnie N (ORCID: 0000-0002-7069-893X)
Publisher PANGAEA
Publication Year 2024
Funding Reference Danish National Research Foundation https://doi.org/10.13039/501100001732 Crossref Funder ID DNRF145 Danish Center for Hadal Research, HADAL
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Resource Type Dataset
Format text/tab-separated-values
Size 234 data points
Discipline Earth System Research
Spatial Coverage (-177.291W, -31.936S, -176.117E, -29.228N); Kermadec Trench
Temporal Coverage Begin 2017-12-06T08:28:01Z
Temporal Coverage End 2017-12-09T12:57:01Z