δ¹⁵N in planktonic foraminifera species Globigerina bulloides and Globorotalia inflata from IODP Site 361-U1475

DOI

Individual species were identified and ~550 individuals per species were picked from the >250 µm fraction under dissecting microscope for each sample. Approximately 7 mg of picked and identified foraminifera shells were crushed between glass microscope slides and rinsed with MilliQ water. Samples were cleaned and prepared for δ¹⁵NFB measurement as in Smart et al. (2020, doi:10.1029/2019gc008440). Nitrate concentrations were measured by chemiluminescence on a Teledyne Instruments (Model 200E) chemiluminescence NO/NOx analyzer (Braman & Hendrix, 1989; doi:10.1021/ac00199a007). δ¹⁵NFB was measured by bacterial conversion of nitrate to nitrous oxide (Sigman et al., 2001; doi:10.1021/ac010088e), with measurement of the δ¹⁵N of the nitrous oxide by automated extraction and gas chromatography-isotope ratio mass spectrometry (Casciotti et al., 2002; doi:10.1021/ac020113w ) on a Thermo Delta V Plus IRMS. The potassium nitrate reference materials IAEA-N3 and USGS 34 (+4.7 ‰ and 1.8 ‰, respectively) were used to standardize results (Gonfiantini et al., 1995). Sample replicates and triplicates were analyzed when possible. Full procedural replicates were analyzed for 161 sample splits, representing 77 unique samples, when enough foraminifera were available for duplicate or triplicate analysis. Precision for full procedural replicates was 1.0 ‰, this is comparable with modern shell-bound measurements of the same species taken from net tows in this region (standard error = 0.9 ‰, n=10 & 1.1 ‰, n=6, of G. bulloides and G. inflata, respectively; Smart et al., 2020). In every batch, full operational blanks and amino acid standards (USGS 65 amino acid standard) were used to correct for the persulfate oxidation blank and to ensure complete conversion of N. The age model is based on the benthic oxygen isotope stratigraphy presented by Starr et al. (2021, doi:10.1038/s41586-020-03094-7).

Identifier
DOI https://doi.org/10.1594/PANGAEA.945839
Related Identifier IsPartOf https://doi.org/10.1594/PANGAEA.946021
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.945839
Provenance
Creator Marcks, Basia ORCID logo; Santos, Thiago Pereira dos ORCID logo; Lessa, Douglas Villela de Oliveira ORCID logo; Cartagena-Sierra, Alejandra (ORCID: 0000-0002-0856-077X); Berke, Melissa A ORCID logo; Starr, Aidan ORCID logo; Hall, Ian R ORCID logo; Kelly, Roger P; Robinson, Rebecca S ORCID logo
Publisher PANGAEA
Publication Year 2023
Funding Reference National Science Foundation https://doi.org/10.13039/100000001 Crossref Funder ID 1737218 Tracking the Subtropical Front across the Mid-Pleistocene Transition Using Sea Surface Temperature and Nutrients
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Representation
Resource Type Dataset
Format text/tab-separated-values
Size 1453 data points
Discipline Earth System Research
Spatial Coverage (25.260W, -41.427S, 25.261E, -41.427N)
Temporal Coverage Begin 2016-02-24T01:55:00Z
Temporal Coverage End 2016-02-27T04:15:00Z