Dynamics of amyloid protein fibril growth using isotope-labelled SANS

DOI

This proposal aims use a novel approach whereby deuterated amyloid peptide fibrils are givenhydrogenated growing ends, and analysed using SANS. The data will enable us to measureindependently both fibril length and linear growth rates for amyloid fibrils. This cannot be doneusing current biophysical techniques, which only probe the rates of conversion of overall %peptide in non-fibrillar to fibrillar form; our proposed experiments absolutely depend on the ability to exploit the contrast between hydrogenated and deuterated peptides that neutron scattering allows. Linear growth rate information is key to understanding the physico-chemical and biological properties of amyloid fibrils, in processes ranging from prion disease propagation, to self-assembly of artificial peptide nano-fibers, and our method will greatly enhance research in the field.

Identifier
DOI https://doi.org/10.5286/ISIS.E.24088071
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24088071
Provenance
Creator Dr Cameron Neylon; Dr Adam Squires; Ms Gudrun Lotze; Dr Annela Seddon
Publisher ISIS Neutron and Muon Source
Publication Year 2014
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2011-10-28T08:29:54Z
Temporal Coverage End 2011-10-29T17:56:49Z