Temperature response curves of Emiliania huxleyi during laboratory experiments


At the end of the two year incubation of a subantarctic E. huxleyi culture (2015-11-20 to 2017-12-01), a temperature response experiment was performed using the Now (Day 747) and Future (Day 725) populations. Now and Future populations were grown in triplicate 28 ml Nalgene Oak Ridge-style centrifuge tubes (Sigma) in Now or Future medium, respectively. The tubes were held in an aluminium temperature gradient block similar to Thomas et al. (1963), that was heated at one end and cooled at the other using water maintained at constant temperature by refrigerated circulators (Julabo GmbH). The temperature regime resulted in a range of 10 temperatures along the length of the block from 6.2°C at one end to 16.5°C at the other, with a range within replicates at each temperature of 0.1 – 0.2°C. The block was lit from below which achieved a light level of 62 +/- 7 μmol m⁻² s⁻¹ at the bottom of the tubes in a 12 hour: 12 hour light: dark cycle. To ensure uniform light each replicate was manually rotated through the row holes at the set temperature during the incubation period. Measurements of in vivo chl-a were made daily and growth rates calculated from the least squares regression of the natural logarithm of in vivo fluorescence, versus time.

The study was supported by Coasts and Oceans Centre (Strategic Science Investment Fund of the National Institute of Water and Atmospheric Research).

DOI https://doi.org/10.1594/PANGAEA.959835
Related Identifier IsPartOf https://doi.org/10.1594/PANGAEA.959857
Related Identifier IsSupplementTo https://doi.org/10.1371/journal.pone.0284415
Related Identifier References https://doi.org/10.4319/lo.1963.8.3.0357
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.959835
Creator Armstrong, Evelyn (ORCID: 0000-0001-8143-027X); Law, Cliff S ORCID logo
Publisher PANGAEA
Publication Year 2023
Funding Reference University of Otago https://doi.org/10.13039/100008247 Crossref Funder ID ORF_0115-0316_Law University of Otago Research Grant; University of Otago https://doi.org/10.13039/100008247 Crossref Funder ID ORF_0117-0318_Law University of Otago Research Grant
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Resource Type Dataset
Format text/tab-separated-values
Size 297 data points
Discipline Design; Fine Arts, Music, Theatre and Media Studies; Humanities
Spatial Coverage (171.532 LON, -45.829 LAT)