Probing membrane localisation of molecular rotors (NR)

DOI

A number of important biological processes, such as intracellular transport and signal transduction depend on the viscosity and the distribution of microdomains in cells. We have recently suggested a novel method of imaging viscosity in single live cells based on fluorescence detection from small fluorophores called molecular rotors. One of our major goals is to create a rotor suitable for probing viscosity inside a plasma membrane of living cells. So far, we have designed two classes of suitable chromophores: BODIPY rotors and conjugated porphyrin dimers. This proposal will use neutron reflection (NR on SURF instrument) to determine the precise positioning of 5 membrane-soluble molecular rotors with respect to floating bi-layer structure, using deutrated lipid DPPC.

Identifier
DOI https://doi.org/10.5286/ISIS.E.24081486
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24081486
Provenance
Creator Dr Marina Kuimova
Publisher ISIS Neutron and Muon Source
Publication Year 2014
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2011-03-19T11:19:24Z
Temporal Coverage End 2011-03-21T08:49:38Z