Probing membrane localisation of molecular rotors (NR)


A number of important biological processes, such as intracellular transport and signal transduction depend on the viscosity and the distribution of microdomains in cells. We have recently suggested a novel method of imaging viscosity in single live cells based on fluorescence detection from small fluorophores called molecular rotors. One of our major goals is to create a rotor suitable for probing viscosity inside a plasma membrane of living cells. So far, we have designed two classes of suitable chromophores: BODIPY rotors and conjugated porphyrin dimers. This proposal will use neutron reflection (NR on SURF instrument) to determine the precise positioning of 5 membrane-soluble molecular rotors with respect to floating bi-layer structure, using deutrated lipid DPPC.

Metadata Access
Creator Dr Marina Kuimova
Publisher ISIS Neutron and Muon Source
Publication Year 2014
Rights CC-BY Attribution 4.0 International;
OpenAccess true
Contact isisdata(at)
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2011-03-19T11:19:24Z
Temporal Coverage End 2011-03-21T08:49:38Z