Effect of formulation method and lipid composition on liposome lamellarity


Liposomes are vesicles comprising a phospholipid membrane bilayer around an aqueous core. They are well-established in the fields of drug delivery and diagnostics. To translate formulations from bench to bedside, their structure and morphology must be precisely understood. Specifically, characterising lamellarity (number of bilayers) in vesicle populations is crucial as it influences release kinetics and encapsulation efficiency. We have characterised the effect of formulation method and lipid composition on vesicle lamellarity in phosphocholine vesicles. Here, we propose applying these models to study phospholipid-based vesicles which more closely represent those in pharmaceutical formulations, which include cholesterol, and positively and negatively charged lipids. The results are highly significant when designing vesicle formulations for applications in diagnostics and drug delivery.

DOI https://doi.org/10.5286/ISIS.E.RB1920464-1
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/109730736
Creator Dr Michael Thomas; Dr Maggie Holme; Miss Valeria Nele; Dr Hanna Barriga; Dr James Doutch; Professor Molly Stevens; Dr Jelle Penders; Miss Cat Saunders; Miss Nayoung Kim; Mr Michael Potter
Publisher ISIS Neutron and Muon Source
Publication Year 2023
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Resource Type Dataset
Discipline Biology; Biomaterials; Chemistry; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering; Natural Sciences; Physics
Temporal Coverage Begin 2020-03-08T22:29:27Z
Temporal Coverage End 2020-03-11T08:30:00Z