Environmental Surveillance of ESBL and Carbapenemase-Producing Gram-Negative Bacteria in a hospital in Ghana

We investigated the presence and prevalence of ESBL- and Carbapenemase-carrying bacterial species by swabbing "high touch" areas as deduced from literature (taps, door handles, switches, etc.) in four wards in the Cape Coast Teaching Hospital. We recovered bacterial growth from all swabs plated on agar in the absence of antimicrobial selection. We compared this bacterial growth on plates containing vancomycin + either ertapenem or cefotaxime inoculated in parallel: identifying species by MALDI-ToF spectra and ESBL and carbapenemase presence through PCR. The high prevalence of A. baumannii carrying blaNDM-1 we observed was further characterised by whole genome sequencing and phylogenetic analysis to determine the relationship between resistant strains found in different wards. Our study, therefore, sought to address this concern and bridge the gap in research literature relating to surveillance of ESBL and carbapenemase-producing gram-negative AMR pathogens in hospital environments to advise IPC practices and aid in setting research priority agenda in Ghana.

Identifier
Source https://data.blue-cloud.org/search-details?step=~012DD412F910CEA0EFF007CDD3ADFAD48471622E839
Metadata Access https://data.blue-cloud.org/api/collections/DD412F910CEA0EFF007CDD3ADFAD48471622E839
Provenance
Instrument Illumina MiSeq; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Temporal Point 2021-01-01T00:00:00Z