Isopods are a diverse group of crustaceans, that inhabit various environments, including terrestrial, freshwater, and marine, both on the surface and in the underground. To unravel the molecular basis of their adaptability, especially to cave habitat, we generated a comprehensive RNAseq dataset comprising representatives from both cave and surface environments belonging to the three different suborders (Asellota, Sphaeromatidea and Oniscidea).