The main objective of this study was to investigate the suitability of mixture of activated carbon and marine sediment as a bioanode in a plant-MFC system with Spartina anglica. Here it was studied how different mixtures of the activated carbon (AC) and the marine sediment (MS) as an anode material affected the plant vitality, electricity generation and spatial microbial community. Four different anode compositions were used to fill the anode compartments (650ml). Plant-MFC 1 and Plant-MFC 2, this duplicate was named as AC100, were filled with 100% activated carbon (AC); Plant-MFC 3 and Plant-MFC 4, this duplicate was named as MS100, were only filled with marine sediment; Plant-MFC 5 and Plant-MFC 6, this duplicate was named as AC67, were filled with a mixture of 67 % AC and 33% marine sediment; and Plant-MFC 7 and Plant-MFC 8, this duplicate was named as AC33, were filled with a mixture of 33% AC and 67% marine sediment.