Multiplexed ISSR genotyping by sequence (MIG-seq) with non-model organisms

We developed a PCR-based procedure for constructing reduced representation libraries without RE digestion steps, de novo SNP discovering, and its genotyping with NGS platform. By using multiplexed inter-simple sequence repeat (ISSR) primers, thousands of genome-wide regions were effectively amplified from a wide variety of genomes without prior genetic information. To validate and demonstrate this method, we sequenced 84 genomic libraries from eight non-model species (a fungus, three plants, three invertebrate and a vertebrate).

Identifier
Source https://data.blue-cloud.org/search-details?step=~0121F0C4161A90DFF873EF5D1A4A714C418548FC2C4
Metadata Access https://data.blue-cloud.org/api/collections/1F0C4161A90DFF873EF5D1A4A714C418548FC2C4
Provenance
Instrument Illumina MiSeq; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Spatial Coverage (127.680W, 26.210S, 142.200E, 45.420N)
Temporal Coverage Begin 2010-01-01T00:00:00Z
Temporal Coverage End 2014-01-01T00:00:00Z