Flow cytometric assessment of virus-like particle, prokaryote and small phototrophic eukaryote abundances from sea foam, surface microlayer, 1-m depth, rainwater from the Swedish Skagerrak (Feb 2020)


Flow cytometry samples for analysis of virus-like particle, prokaryote and small phototrophic eukaryote abundances were collected from sea foam, surface microlayer and 1-m deep water from the Swedish Skagerrak during a 1-month campaign at Tjärnö Marine Laboratory in February 2020. Using a small boat, foam and microlayer were collected using a glass plate sampler, and 1-m deep water using a syringe connected to a hose. In addition, rainwater for flow cytometry was collected close to the coast in Duran bottles. Samples were fixed with glutardialdehyde (1% final concentration, Merck, Sweden), stored for 1 hour in the dark and subsequently stored at -80 °C. The purpose of the cellular and viral abundance assessment was to complement metagenomic analyses to investigate viral dispersal across the air-sea interface and along the natural water cycle. In addition, metadata for light conditions, wind speed, salinity, and temperature were recorded. For further details on the campaign and flow cytometric analysis, please refer to Rahlff et al., 2022.

DOI https://doi.org/10.1594/PANGAEA.947348
Related Identifier References https://doi.org/10.1038/s41467-023-42125-5
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.947348
Creator Rahlff, Janina ORCID logo; Esser, Sarah P ORCID logo; Heinrichs, Mara Elena; Giebel, Helge-Ansgar ORCID logo; Probst, Alexander J ORCID logo
Publisher PANGAEA
Publication Year 2023
Funding Reference European Commission https://doi.org/10.13039/501100000780 Crossref Funder ID 730984 https://cordis.europa.eu/project/id/730984 Association of European Marine Biological Laboratories Expanded; German Aerospace Center https://doi.org/10.13039/501100002946 Crossref Funder ID 50WB1922 https://foerderportal.bund.de/foekat/jsp/SucheAction.do?actionMode=view&fkz=50WB1922 DISPERS - Verbreitung von Mikroorganismen und Viren zur Antarktis mit Auswirkungen auf die Weltraumforschung; German Research Foundation https://doi.org/10.13039/501100001659 Crossref Funder ID 34509606 https://gepris.dfg.de/gepris/projekt/34509606 Ökologie, Physiologie und Molekularbiologie der Roseobacter-Gruppe: Aufbruch zu einem systembiologischen Verständnis einer global wichtigen Gruppe mariner Bakterien; German Research Foundation https://doi.org/10.13039/501100001659 Crossref Funder ID 429483359 https://gepris.dfg.de/gepris/projekt/429483359 NOVAC - Neuartige Viren infizieren Archaeen im terrestrischen Untergrund; German Research Foundation https://doi.org/10.13039/501100001659 Crossref Funder ID 446702140 https://gepris.dfg.de/gepris/projekt/446702140 VIBOCAT - Erforschung des Virioneustons: Viral-bakterielle Interaktionen zwischen Ozean und Atmosphäre
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Resource Type Dataset
Format text/tab-separated-values
Size 370 data points
Discipline Biospheric Sciences; Ecology; Geosciences; Natural Sciences
Spatial Coverage (11.106W, 58.876S, 11.146E, 58.887N)
Temporal Coverage Begin 2020-02-03T12:30:00Z
Temporal Coverage End 2020-02-26T14:00:00Z