Aquarium monitoring during a land-based experiment testing the cumulative effect of Cu exposure and ocean acidification on cold-water coral Dentomuricea aff. meteor


We report the results of a land-based experiment testing the cumulative effects of copper (Cu) exposure and ocean acidification (OA). Corals were obtained as by-catch during experimental long-line fisheries campaigns on board R/V "Arquipélago" (ARQDAÇO monitoring program, University of the Azores) at Baixa de São Mateus at 185 m depth in April 2019. The experiment was undertaken at the DeepSeaLab aquaria facilities (Okeanos-University of the Azores) where corals were exposed to four OA/Cu-contamination scenarios: (1) ambient pCO2/pH level as measured in situ conditions (385 μatm/ pH 8.09); (2) high pCO2/reduced pH (IPCC RCP8.5 scenario, 1000 μatm/ pH 7.73); (3) ambient pCO2/pH level and additional Cu concentration (60 µg/l); (4) high pCO2/reduced pH and additional Cu concentration (60 µg/l). The pH/pCO2 modification was achieved by bubbling seawater with either pure CO2 (to increase pCO2) or CO2 low air (to decrease pCO2). The copper concentration used in this experiment followed a trial simulating a polymetallic particles plume release during a potential deep-sea mining event. This sublethal concentration was found to be the highest copper concentration dissolved in seawater.Seawater physical-chemical parameters were measured daily in each chamber. Temperature, pH and oxygen saturation were measured manually in each aquaria every day using a Mettler-Toledo S8 glass and a Fibox4 (PreSens) with an Oxygen Probe PSt3.

Species: octocoral Dentomuricea aff. meteor1. Experimental treatmentsTreatments8.1 + [0]: ambient pH plus no copper addition8.1 + [60]: ambient pH plus Cu concentration of 60 μg/l7.7 + [0]: low pH plus no copper addition7.7 + [60]: low pH plus Cu concentration of 60 μg/lExperiment duration: 9 days

Metadata Access
Creator Martins, Ines ORCID logo; Goulart, Joana; Godinho, Antonio; Mano, Beatriz; Sire de Vilar, Anaïs ORCID logo; Carreiro-Silva, Marina ORCID logo
Publisher PANGAEA
Publication Year 2024
Funding Reference European Commission Crossref Funder ID ACORES-01-0145-FEDER-000122 ACORES 2020; European Commission Crossref Funder ID ACORES-01-0145-FEDER-000140 InvestigadoresMarAZ-Consolidar corpo de investigadores em Ciências do Mar nos Açores; Horizon 2020 Crossref Funder ID 818123 Integrated Assessment of Atlantic Marine Ecosystems in Space and Time
Rights Creative Commons Attribution 4.0 International; Data access is restricted (moratorium, sensitive data, license constraints);
OpenAccess false
Resource Type Dataset
Format text/tab-separated-values
Size 2748 data points
Discipline Earth System Research
Spatial Coverage (-29.083W, 38.250S, -28.270E, 38.900N); Condor Seamounts; Azores
Temporal Coverage Begin 2019-04-30T00:00:00Z
Temporal Coverage End 2019-10-10T00:00:00Z