Comparison of three bisulfite sequencing methods on two reef building corals

Genome-wide DNA methylation studies using bisulfite sequencing have been hampered by high costs and potential biases due to methods in non-model organisms. To improve understanding of differences in the bisulfite sequencing methods and ultimately how genome-wide DNA methylation studies are carried out, we compared three methods for quantifying DNA methylation at single base pair resolution: Whole Genome Bisulfite Sequencing (WGBS), Reduced Representation Bisulfite Sequencing (RRBS), and Methyl-CpG Binding Domain Bisulfite Sequencing (MBDBS). We used replicate individuals from two reef building coral species with contrasting environmental sensitivity, the more resistant Montipora capitata and the more sensitive Pocillopora acuta.

Identifier
Source https://data.blue-cloud.org/search-details?step=~0124D96E2736466E5C406318A271E56BD13D67FE0E4
Metadata Access https://data.blue-cloud.org/api/collections/4D96E2736466E5C406318A271E56BD13D67FE0E4
Provenance
Instrument Illumina NovaSeq 6000; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Spatial Coverage (-157.835W, 21.430S, -157.790E, 21.475N)
Temporal Coverage Begin 2018-09-04T00:00:00Z
Temporal Coverage End 2018-09-07T00:00:00Z