Interaction of soil amoeba Protostelium aurantium with three pathogenic yeasts Candida spp. (here: C. parapsilosis)

We investigated different escape and defense strategies of Candida parapsilosis against the natural fungivorous predator Protostelium aurantium using dual RNA-Seq. Trophozoites of the amoeba Protostelium aurantium were fed with C. parapsilosis and samples for RNA isolation were taken at time points 0 min (control), 30 min and 60 min. At indicated time points samples were shock frozen and used for RNA isolation. C. parapsilosis underwent rapid predation and intracellular killing of the yeast Overall design: Analysis of nine samples in total. Three time points (0min, 30min and 60min) with three replicates each. The three 0min samples are the control samples.

Identifier
Source https://data.blue-cloud.org/search-details?step=~012EF3AF453CF2E41A30D57FB0F927524CE85E67D27
Metadata Access https://data.blue-cloud.org/api/collections/EF3AF453CF2E41A30D57FB0F927524CE85E67D27
Provenance
Instrument Illumina MiSeq; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Contributor Evolution of Microbial Interactions, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute (HKI)
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Temporal Point 2018-09-26T00:00:00Z