TemBi 2014 mesocosm study: Summer storm impact on size-fractionated bacterial protein production and abundance of heterotrophic prokaryotes and picocyanobacteria


We simulated an experimental summer storm in large-volume (~1200 m3, ~16m depth) enclosures in Lake Stechlin (https://www.lake-lab.de) by mixing deeper water masses from the meta- and hypolimnion into the mixed layer (epilimnion). The mixing included the disturbance of a deep chlorophyll maximum (DCM) which was present at the same time of the experiment in Lake Stechlin and situated in the metalimnion of each enclosure during filling. Size-fractionated Bacterial Protein Production (BPP) of particle associated (PA, >3.0 µm) and free-living bacteria (FL, 0.2-3.0 µm) (14C-Leu incorporation) as well as abundances of PA (microscopy of DAPI stained cells on 3.0 µm polycarbonate filters) and FL heterotrophic prokaryotes and picocyanobacteria (flow cytometry of SYBR green I stained cells) were monitored for 42 days after the experimental disturbance event. Mixing increased bacterial abundance and production about 3 weeks after mixing, which was associated to a mixing-induced stimulation of phytoplankton growth in the mixed enclosures compared to the controls. Simultaneously, decreased abundances of picocyanobacteria could be observed in mixed enclosures.

Empty cells = NAFurther Project information: Core Facility grant; Award: GE 1775/2-1

DOI https://doi.org/10.1594/PANGAEA.930921
Related Identifier IsPartOf https://doi.pangaea.de/10.1594/PANGAEA.931002
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.930921
Creator Hornick, Thomas ORCID logo; Mach, Elke; Grossart, Hans-Peter ORCID logo
Publisher PANGAEA
Publication Year 2021
Funding Reference Federal Ministry of Education and Research https://doi.org/10.13039/501100002347 Crossref Funder ID 01LC1501G Bridging in Biodiversity Science; Federal Ministry of Education and Research https://doi.org/10.13039/501100002347 Crossref Funder ID 033L041B Stickstofflimitation in Binnengewässern; Leibniz Association https://doi.org/10.13039/501100001664 Crossref Funder ID SAW-2011-IGB-2 Climate driven Changes in Biodiversity of Microbiota
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Resource Type Dataset
Format text/tab-separated-values
Size 2003 data points
Discipline Earth System Research
Spatial Coverage (13.028 LON, 53.143 LAT); Germany
Temporal Coverage Begin 2014-08-04T00:00:00Z
Temporal Coverage End 2014-09-16T00:00:00Z