Understanding the antimicrobial activity of the salivary protein Histatin 5

DOI

The goal of this project is to understand the antimicrobial activity of the intrinsically disordered salivary protein Histatin 5, which acts in defence against oral candidiasis caused by Candida albicans. From the literature it is still unknown how the peptide transports itself through the membrane, but it is suggested to not be an ordinary pore-opening transport, instead it has been attributed to its metal binding abilities. Studies have established that various transitional metals, such as Zn, Ni, Cu, and Fe, are intrinsically present in the saliva. In this project, we aim to investigate not only how Histatin 5 interacts with the SiO2 surface but also how Histatin 5 interacts with membranes, to be able to detect structural changes of the membrane, as well as determine how Histatin 5 inserts into the membrane. For this purpose, a combined experimental and theoretical approach is used.

Identifier
DOI https://doi.org/10.5286/ISIS.E.87839919
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/87839919
Provenance
Creator Dr Sandeep Chakane; Miss Stéphanie Jephthah; Professor Marie Skepö; Miss Carolina Cragnell; Dr Yuri Gerelli; Dr Rebecca Welbourn
Publisher ISIS Neutron and Muon Source
Publication Year 2021
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Chemistry; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering; Natural Sciences
Temporal Coverage Begin 2018-10-01T08:00:00Z
Temporal Coverage End 2018-10-04T10:58:02Z