Developing platforms for membrane protein studies -nanodiscs at solid-liquid interfaces

DOI

The nanodisc (ND) is a self-assembled discodial particle composed of phospolipids and two amphipathic belt proteins, termed Membrane Scaffold Proteins (MSP). This stable, monodispersed nanometer-sized particle (a diameter of 10 nm and a height of ~5 nm), each resembling a piece of a lipid bilayer, provides a native-like model of the cell membrane. A single membrane protein can be reconstituted into the nanodisc, which are a promising tool in studies of membrane proteins in their functional state. We have preliminary QCM data which shows we can assemble nanodisks onto grafted phosphocholine surfaces, and in principle, this could result is a nanodisc layer 'floating' on a cushion of water, analogous to floating lipid bilayers. This could potentially be a very useful system for studying membrane proteins. In this experiment, we wish to characterise the structure of these adsorbed layers.

Identifier
DOI https://doi.org/10.5286/ISIS.E.55225366
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/55225366
Provenance
Creator Ms Vivien Jagalski; Dr Rob Barker; Professor Marité Cárdenas Gómez; Dr Arwel Hughes
Publisher ISIS Neutron and Muon Source
Publication Year 2017
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2014-08-04T01:00:00Z
Temporal Coverage End 2014-08-07T01:00:00Z