Raw sequence data from sorted CySCS in control and Rbf knockdown using Tj>GFP for sorting.

DOI

Sequencing reads for sorted cells using Tj-Gal4, UAS-GFP to sort CySCs and early cyst cells in the Drosophila testis. Data are for 3 replicates of control (Tj>GFP) and 3 replicates of Rbf knockdown (Tj>GFP, Rbf RNAi), labelled "control" and "condition", respectively. Cells were isolated by FACS and RNA extracted and amplified using a Clontech SmartSeq v4 kit, before library preparation and Illumina sequencing.Reads for each sample are split into 4 FASTA files. The file names for the controls (Tj>GP) begin with "3-Control1", "3-2-Control2", and "3-3-Control3". The Rbf knockdowns are labelled "4-Rbi1", "4-2-Rbi2" and "4-3-Rbi3".

Identifier
DOI https://doi.org/10.5522/04/13484814.v1
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Metadata Access https://api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:figshare.com:article/13484814
Provenance
Creator Amoyel, Marc; Sainz De La Maza Redondo, Diego
Publisher University College London UCL
Contributor Figshare
Publication Year 2021
Rights https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact researchdatarepository(at)ucl.ac.uk
Representation
Language English
Resource Type Dataset
Discipline Basic Biological and Medical Research; Biochemistry; Biology; Cell Biology; Developmental Biology; Life Sciences; Medicine