The SESAME dataset contains mesozooplankton data collected during April 2008 in the North-Western part of Black Sea (between 44°46' N and 42°29'N latitude and 28°64'E and 30°59'E longitude). Mesozooplankton sampling was undertaken at 9 stations where samples were collected using a Hensen net in the 0-10, 10-25, 25-50, 50-100, 100-150, 150-200 m layer. The dataset includes 29 samples analysed for mesozooplankton species composition and abundance. The entire sample or an aliquot (1/2 to 1/4) was analyzed under the binocular microscope. Calculations of zooplankton abundance are made by the following formulae, in accordance with the Report of the third ICES/HELCOM workshop on quality assurance of Biological measurements Warnemünde, Germany, 1996. M - number of counted specimens (ind.), Vf - volume of filtrated water (m³), and K - counted part of sample. (http://www2008.io-warnemuende.de/research/helcom_zp/documents/qa_zp_part.pdf)