Neutron reflectivity studies of the interaction between novel peptide antibiotics and model bacterial membranes

DOI

There is an urgent need for development of novel antimicrobial therapies, and the mammalian theta-defensins and protegrins seem to offer significant potential in this regard. These peptides are crosslinked by multiple disulfide bonds and - in the case of the defensins - are also cyclised through their N- and C-termini. Both exhibit potent antibacterial activity, but differ in the extent of their side effects in terms of red blood cell lysis. Neutron reflectivity studies will be performed to characterise the interaction between these two peptides and model bacterial cell membranes. The peptides for study include the Rhesus monkey theta-defensin, RTD-1, and the porcine protegrin, PG-1. Interaction of these two peptides with a model bacterial membrane will be characterised using a floating phospholipid bilayer supported above a gold-coated substrate with covalently bound thioalkyl monolayer

Identifier
DOI https://doi.org/10.5286/ISIS.E.47623272
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/47623272
Provenance
Creator Professor Jayne Lawrence; Dr Arwel Hughes; Dr Dave Barlow; Dr Ivana Knyght; Mr Yussif Saaka
Publisher ISIS Neutron and Muon Source
Publication Year 2017
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2014-03-02T00:00:00Z
Temporal Coverage End 2014-03-03T00:00:00Z