Picoeukaryote counts determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities


Two Fe-Mn bottle amendment experiments with two natural phytoplankton communities were performed during Polarstern expedition PS97 in 2016 in the Drake Passage. At two locations, sea water was pumped (using trace metals clean techniques) from 25m depth and used to fill polycarbonate bottles after having passed through a cleaned 200 μm mesh (removing large grazers). The Control treatment was the sampled seawater without any trace metals addition while the other three treatments were enriched with either FeCl3 alone (0.5 nM; +Fe treatment) or MnCl2 alone (1 nM; +Mn treatment) or both trace metals together (+FeMn treatment). All treatments were done in triplicate 2,5L PC bottles. All incubation bottles were maintained at 30 μmol photons m-2 s-1 under a 16:8 (light:dark) hour cycle at 1 ̊C.Autotrophic picoeukaryotes were analyzed via flow cytometry. At the start and the end of the experiments, samples were preserved with 10% buffered formalin, flash-frozen in liquid nitrogen, and analyzed flow cytometrically to assess picoplankton densities. Before running the samples, 2 μL beads (Sperotech - Rainbow Fluorescent Particles (RFPs) - 2.11 μm) were added to each treatment as a size and fluorescence reference. Then picoeukaryotes were identified based on side scatter versus FL-3. Three P subgroups (0.2 – 2 μm) were differentiated according to their size : small (P1), medium (P2) and large (P3), according to sub-cluster of events.

DOI https://doi.org/10.1594/PANGAEA.935862
Related Identifier IsPartOf https://doi.org/10.1594/PANGAEA.935868
Related Identifier References https://doi.org/10.1038/s42003-022-03148-8
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.935862
Creator Balaguer, Jenna ORCID logo; Koch, Florian ORCID logo; Hassler, Christel S ORCID logo; Trimborn, Scarlett ORCID logo
Publisher PANGAEA
Publication Year 2021
Funding Reference German Research Foundation https://doi.org/10.13039/501100001659 Crossref Funder ID 5472008 https://gepris.dfg.de/gepris/projekt/5472008 Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Resource Type Dataset
Format text/tab-separated-values
Size 298 data points
Discipline Earth System Research
Spatial Coverage (-66.362W, -60.413S, -60.865E, -58.869N); Drake Passage; Scotia Sea
Temporal Coverage Begin 2016-03-02T08:19:00Z
Temporal Coverage End 2016-03-17T11:16:00Z