This study explored the variation of ileal endogenous amino acids (IEAAs) losses and its influencing factors in chickens offered nitrogen-free diet (NFD) contained different ratios of amylose and amylopectin (AM/AP). A total of 252, 28-d-old AA broiler chickens were randomly allocated into 7 treatment groups for a 3-d trial. The dietary treatments included a basal diet (control), a NFD containing corn starch (CS), and five NFD with AM/AP ratios were 0.20, 0.40, 0.60, 0.80, and 1.00, respectively.