Marine Isotope Stage (MIS) M2, 3.3 Ma, is an isolated cold stage punctuating the benthic oxygen isotope (δ¹⁸O) stratigraphy of the warm Piacenzian interval of the late Pliocene Epoch. The prominent (~0.65‰) δ¹⁸O increase that defines MIS M2 has prompted debate over the extent to which it signals an early prelude to the rhythmic extensive glaciations of the northern hemisphere that characterise the Quaternary and raised questions about the forcing mechanisms responsible. Recent work suggests that CO₂ storage in the deep Atlantic Ocean played an important role in these events but detailed reconstructions of deep ocean chemical stratification are needed to test this idea and competing hypotheses. Here we present new records of the Nd isotope composition of fish debris and δ¹³C and B/Ca ratios of benthic foraminifera from the northwest and southeast Atlantic Ocean. Our novel geochemical data show that, in contrast to major Quaternary glaciations such as MIS 2 (~21 ka) and MIS 100 (~2.52 Ma), the deep North Atlantic Ocean was weakly chemically stratified during MIS M2. We show that Southern Component Water incursion into the Atlantic Ocean was limited to the deep South Atlantic basin during MIS M2 and peaked well before (~10-15-kyr) the atmospheric CO₂ minimum. Our findings imply that the deep Atlantic Ocean was not the principle sink of CO₂ sequestered from the atmosphere during MIS M2, implicating a different CO₂ storage deep-water reservoir mechanism, presumably Southern Component Water incursion into the Pacific Ocean. Weak chemical stratification in the deep Atlantic Ocean during MIS M2 relative to MIS 100 and 2 suggests comparatively active Atlantic meridional overturning circulation. That suggestion is consistent with the warmth of the high latitude North Atlantic during MIS M2 - surface water temperatures cooled during M2 but only to Holocene values. Our findings may help to explain the paucity of evidence for extensive early glaciation of the northern hemisphere during M2 but leave open the possibility of ice sheet advance on Antarctica.