Microbe abundance and biomass in the euphotic zone of the Aegean Sea at Station MARCH-1997-GN36199704601MSB01 in spring 1997

DOI

10-30 ml subsamples were concentrated on 25 mm black polycarbonate filters (0.6 µm for NF, 0.2 µm for B), stained with DAPI for 10 min and filtered. NF were counted using UV and blue excitation and enumerated. Heterotrophic B were counted using UV excitation. NF were classified in size categories and biovolume was calculated. NF abundance data were converted into C biomass using 220 fgC µm-3. Bact abund data were converted into C biomass using 20 fgC cell-1. BP C was estimated by the 3H-leucine approach according to Kirchman et al. (1986) and Kirchman D.L., 1993. Leucine incorporation as a measure of biomass production by heterotrophic bacteria. In: Kemp P.F. et al. (eds), 1993. Handbook of methods in aquatic microbial ecology. Lewis Publishers, Ann Arbor, 509-512). At each depth duplicate samples and a control one were incubated with 1 nM L-[4,5 3H]-leucine (specific activity 128 Ci/mmol) and 18 nM non-radioactive leucine. Samples were incubated in the dark at in situ temperature.

Identifier
DOI https://doi.org/10.1594/PANGAEA.678539
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.678539
Provenance
Creator Christaki, Urania ORCID logo; Van Wambeke, France ORCID logo; Giannakourou, Antonia ORCID logo
Publisher PANGAEA
Contributor Université du Littoral Côte d'Opale
Publication Year 2008
Funding Reference Fourth Framework Programme https://doi.org/10.13039/100011105 Crossref Funder ID IC21960002 https://cordis.europa.eu/project/id/IC21960002 Mass Transfer and Ecosystem Response; Fourth Framework Programme https://doi.org/10.13039/100011105 Crossref Funder ID MAS3960051 https://cordis.europa.eu/project/id/MAS3960051 Mass Transfer and Ecosystem Response; Sixth Framework Programme https://doi.org/10.13039/100011103 Crossref Funder ID 36949 https://cordis.europa.eu/project/id/36949 Southern European Seas: Assessing and Modelling Ecosystem Changes
Rights Creative Commons Attribution 3.0 Unported; https://creativecommons.org/licenses/by/3.0/
OpenAccess true
Representation
Resource Type Dataset
Format text/tab-separated-values
Size 30 data points
Discipline Earth System Research
Spatial Coverage (25.100 LON, 35.745 LAT)