This dataset includes the analysis of sediment core DSDP 516 (30°16'S, 35°17'W, 1313 m water depth) recovered from the Rio Grande Rise within the modern day core of Antarctic Intermediate Water (AAIW). It contains benthic foraminiferal stable oxygen and carbon isotopes measured on Cibicidoides wuellerstorfi from the >250μm fraction of DSDP 516 covering the last 600,000 years. The dataset also includes measured reference material Carrara Marble. Measurements were made on a ThermoScientific MAT 253 mass spectrometer coupled with a KIEL IV Carbonate precipitation device.