We have recently used Polref in to measure the assembly of tethered floating lipid bilayers. These are useful models of biological membranes in which a phospholipid bilayer is tethered to a solid surface by a molecular linker. The length of this linker can control the width of the water gap between the surface and the floating bilayer. Since they are easy to make and characterise , they promise an additional method to help us build accurate models of bacterial outer membranes. Here the water gap represents an important cellular compartment called the periplasm. Our previous POLREF experiments showed that we can assemble bilayers at very low tether densities which is important to minimise their effect on the membrane. Here we wish to show that we can insert and characterise proteins within the lipid bilayer which is another important step in creating useful models of living membranes