Mixed greywater (laundry, dishwashing and shower) was collected from a rural household of Noungou, Burkina Faso. The collected greywater was treated in situ through a horizontal constructed wetland. The treatment system is a subsurface horizontal flow wetland with an upstream pre-treatment step. The planted section is semi-underground and made of concrete and planted with Chrysopogon zizanioides. The treated greywater is collected in an underground storage reservoir. Once a week, the raw greywater and treated samples from each section were collected for analysis. The samples were collected in sterile 500 ml glass bottles for microbiological analysis and 1000 ml plastic bottles for physico-chemical analyses. Physico-chemical parameters such as pH, electrical conductivity (EC), temperature (T, °C), and dissolved oxygen (DO) were measured directly from the influent and effluent using a portable pH/EC/TDS/temperature meter (Hanna Instruments, Romania) and Oxi 3310 (WTW Germany GmbH) (Clesceri et al., 1999). The five-day biochemical oxygen demand (BOD5), chemical oxygen demand (COD), suspended solids (SS), nitrate (NO₃⁻), ammonium (NH₄⁺), orthophosphate (PO₄³⁻) were determined according to the Standard Methods (Clesceri et al., 1999). Fecal coliforms, Esherichia coli, and enterococci were used as fecal indicators to evaluate the removal of microorganisms. The spread plate method and Chromocult Coliform Agar ES medium (Merck KGaA, Germany) were used to evaluate fecal coliform and E. coli (44 °C for 24 h), while Slanetz and Barthley agar medium (Liofilchem srl, Italy) (37 °C for 48 h) was used for enterococci (Clesceri et al., 1999).