Detailed Structure of Phospholipid Bilayers from Multiple Contrast Neutron Reflection Data.

DOI

Floating bilayers are supported phospholipid membranes, which are minimally constrained by the influence of the substrate, and therefore considered to be more realistic than other deposited systems. The technology has been developed over a number of years at ISIS/ILL, and we are now able to make very high quality, essentially defect free membranes, repeatably and with a high degree of control over structure and composition. We have recently carried out a detailed structural analysis of a DPPC FSB, and found that the structural parameters are very close to those of membranes measured by other techniques. However, an analysis of the uncertainty of our fitted values shows that some parameters are very poorly determined. In this proposal, we argue that repeating the measurements with differing levels of deuteration should allow us to reduce these uncertainties.

Identifier
DOI https://doi.org/10.5286/ISIS.E.49920183
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/49920183
Provenance
Creator Dr Stephen Holt; Professor Jeremy Lakey; Dr Luke Clifton; Dr Filip Ciesielski; Dr Arwel Hughes
Publisher ISIS Neutron and Muon Source
Publication Year 2017
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2014-06-23T23:00:00Z
Temporal Coverage End 2014-06-25T23:00:00Z