Sediment bacterial community in sandy sediment at CO2 natural vents in Basiluzzo (Panarea, Italy): sediment raw ARISA (Automated Ribosomal Intergenic Spacer Analysis) data

DOI

Sediment bacterial community at natural CO2 vents as described applying molecular fingerprint technique Automated Ribosomal Intergenic Spacer Analysis (ARISA) on sandy sediment samples collected in 2011, 2012 and 2013 collected at Basiluzzo Islet (Panarea Island).Automated ribosomal intergenic spacer analysis (ARISA) is a quantitative fingerprinting approach for estimating the abundance of Operation Taxonomic Units (OUT) in natural microbial communities. A detailed description of this molecular method is provided in Ramette (2009).Briefly PCR-amplified fragments, obtained using primes that yield peaks in the range 100 to 1,000 bp, were separated via capillary electrophoresis and then raw profiles were checked for stable baselines and voltage, and peak sizes and absolute areas were determined by using GeneMapper software v 3.7 (Applied Biosystems) with minimum peak heights of 50 fluorescence units for all dyes. Here the GeneMapper output table is provided. In Molari et al. (XXXX) samples-by-binned-OUT tables was obtained by calculating relative fluorescence intensity (RFI), considering fragments above a threshold of 50 fluorescence units and between 100–1000 bp length, and applying a binning strategy with a bin size of 2 bp.Ramette, Alban (2009). Quantitative Community Fingerprinting Methods for Estimating the Abundance of Operational Taxonomic Units in Natural Microbial Communities. Applied and Environmental Microbiology, 75(8): 2495−2505.

Identifier
DOI https://doi.org/10.1594/PANGAEA.873500
Related Identifier https://doi.org/10.1594/PANGAEA.871453
Related Identifier https://doi.org/10.3289/CR_ECO2_20580
Related Identifier https://doi.org/10.3289/CR_ECO2_23104
Related Identifier https://doi.org/10.3289/CR_ECO2_20593
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.873500
Provenance
Creator Molari, Massimiliano ORCID logo
Publisher PANGAEA
Publication Year 2017
Funding Reference Seventh Framework Programme https://doi.org/10.13039/100011102 Crossref Funder ID 265847 https://cordis.europa.eu/project/id/265847 Sub-seabed CO2 Storage: Impact on Marine Ecosystems
Rights Creative Commons Attribution 3.0 Unported; https://creativecommons.org/licenses/by/3.0/
OpenAccess true
Representation
Resource Type Dataset
Format text/tab-separated-values
Size 1071480 data points
Discipline Earth System Research
Spatial Coverage (15.119W, 38.662S, 15.119E, 38.664N); Panarea
Temporal Coverage Begin 2011-06-05T00:00:00Z
Temporal Coverage End 2013-06-09T00:00:00Z